Journal article
A single point in protein trafficking by Plasmodium falciparum determines the expression of major antigens on the surface of infected erythrocytes targeted by human antibodies
JA Chan, KB Howell, C Langer, AG Maier, W Hasang, SJ Rogerson, M Petter, J Chesson, DI Stanisic, MF Duffy, BM Cooke, PM Siba, I Mueller, PC Bull, K Marsh, FJI Fowkes, JG Beeson
Cellular and Molecular Life Sciences | SPRINGER BASEL AG | Published : 2016
Abstract
Antibodies to blood-stage antigens of Plasmodium falciparum play a pivotal role in human immunity to malaria. During parasite development, multiple proteins are trafficked from the intracellular parasite to the surface of P. falciparum-infected erythrocytes (IEs). However, the relative importance of different proteins as targets of acquired antibodies, and key pathways involved in trafficking major antigens remain to be clearly defined. We quantified antibodies to surface antigens among children, adults, and pregnant women from different malaria-exposed regions. We quantified the importance of antigens as antibody targets using genetically engineered P. falciparum with modified surface antig..
View full abstractGrants
Funding Acknowledgements
Thanks to all participants in the study. We would like to acknowledge Brian Cooke for provision of the 3D7 SBP1KO isolate, Melanie Rug and Alan Cowman for provision of anti-PfEMP3 antibodies and CS2 SBP1KO parasites, Paul Gilson for provision of anti-HSP70 antibodies, Joe Smith for provision of the rabbit anti-DBL3 antibodies, Gaoqian Feng for assistance with opsonic phagocytosis assays and Simon Crawford for assistance with electron microscopy. Funding was provided by the National Health and Medical Research Council of Australia (Program grant and Senior research fellowship to JG Beeson, project grant to JG Beeson and SJ Rogerson, Infrastructure for Research Institutes Support Scheme Grant), Australian Postgraduate Award to J Chan, and the Australian Research Council (Future fellowships to JG Beeson and FJI Fowkes, Australian Research fellowship to AG Maier). The authors gratefully acknowledge support for the Burnet Institute from the Victorian Operational Infrastructure Support Program.